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Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
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Related Experiment Video

Updated: Aug 24, 2025

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
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Liquid-Core Hydrogel Optical Fiber Fluorescence Probes.

Ting Liu1, He Ding1, Jianwei Huang1

  • 1College of Mechanical Engineering and Automation, Huaqiao University, Xiamen, Fujian 361021, China.

ACS Sensors
|October 25, 2022
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel liquid-core hydrogel optical fiber fluorescence probe. This reusable probe offers sensitive, accurate, and selective detection of small-molecule analytes for on-site sensing applications.

Keywords:
Hg2+ detectionfluorescence probehydrogel optical fiberliquid coresmall-molecule analyte detection

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Area of Science:

  • Materials Science
  • Analytical Chemistry
  • Biomedical Engineering

Background:

  • Existing optical fiber fluorescence probes often have complex structures.
  • There is a need for sensitive and selective methods for small-molecule analyte detection, especially for on-site applications.

Purpose of the Study:

  • To report the development and characterization of a novel liquid-core hydrogel optical fiber fluorescence probe.
  • To demonstrate its potential for sensitive and selective small-molecule analyte detection.

Main Methods:

  • Fabrication of a unique optical fiber probe with a liquid core, high-refractive-index hydrogel fiber core, and low-refractive-index hydrogel fiber cladding.
  • Utilizing a sensing solution sealed within the liquid core to react with penetrating small-molecule targets.
  • Quantifying fluorescence signal variations induced by target-analyte interactions.

Main Results:

  • The proposed probe exhibits a unique structural design distinct from conventional probes.
  • Demonstrated high sensitivity, accuracy, and selectivity in practical detection scenarios.
  • The probe is simple, rapid to fabricate, and reusable, indicating cost-effectiveness.

Conclusions:

  • The liquid-core hydrogel optical fiber fluorescence probe represents a novel sensing platform.
  • It offers a promising solution for on-site small-molecule analyte detection challenges.
  • The probe's design and performance pave the way for advanced sensing technologies.