Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Proposed key characteristics of neurotoxic chemicals.

Neurotoxicology·2025
Same author

Introduction to developmental outcomes of neuroinflammatory insults.

Neurotoxicology and teratology·2025
Same author

Assessing replicability and power estimates of behavioral performance of control rats across standardized pre-clinical and toxicology studies.

Neurotoxicology and teratology·2025
Same author

Hyaluronan Ameliorates Viral Pneumonia in Mice and Humans by Inhibiting Transcription Factor E2F1.

American journal of respiratory cell and molecular biology·2025
Same author

hTERT and SV40LgT Renal Cell Lines Adjust Their Transcriptional Responses After Copy Number Changes from the Parent Proximal Tubule Cells.

International journal of molecular sciences·2025
Same author

Proteomic Analysis of Endemic Viral Infections in Neurons offers Insights into Neurodegenerative Diseases.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Aug 24, 2025

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages
06:52

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

Published on: May 21, 2018

10.8K

Imaging Inflammasome Activation in Microglia.

Negin P Martin1, G Jean Harry2

  • 1Neurobiology Laboratory, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina.

Current Protocols
|October 26, 2022
PubMed
Summary

This study presents a new protocol for creating fluorescent inflammasome indicator microglia cells. This method enables researchers to visualize inflammasome activation in real-time and test substances affecting this process.

Keywords:
ASC speckBV-2inflammasomeinflammationmicroglia

More Related Videos

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells
09:04

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Published on: May 22, 2014

21.0K
In Vivo Dynamics of Retinal Microglial Activation During Neurodegeneration: Confocal Ophthalmoscopic Imaging and Cell Morphometry in Mouse Glaucoma
12:48

In Vivo Dynamics of Retinal Microglial Activation During Neurodegeneration: Confocal Ophthalmoscopic Imaging and Cell Morphometry in Mouse Glaucoma

Published on: May 11, 2015

10.7K

Related Experiment Videos

Last Updated: Aug 24, 2025

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages
06:52

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

Published on: May 21, 2018

10.8K
Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells
09:04

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Published on: May 22, 2014

21.0K
In Vivo Dynamics of Retinal Microglial Activation During Neurodegeneration: Confocal Ophthalmoscopic Imaging and Cell Morphometry in Mouse Glaucoma
12:48

In Vivo Dynamics of Retinal Microglial Activation During Neurodegeneration: Confocal Ophthalmoscopic Imaging and Cell Morphometry in Mouse Glaucoma

Published on: May 11, 2015

10.7K

Area of Science:

  • Immunology
  • Neuroscience
  • Cell Biology

Background:

  • Inflammasomes are crucial multiprotein complexes in innate immunity.
  • Inflammasome activation in microglia is linked to neurological disorders.
  • Existing protocols focus on peripheral macrophages, not microglia.

Purpose of the Study:

  • To develop a protocol for generating fluorescent inflammasome indicator microglia cells.
  • To enable real-time imaging of inflammasome activation in microglia.
  • To facilitate the study of substances affecting microglia inflammasome activation.

Main Methods:

  • Utilized recombinant retroviruses to transduce murine BV-2 cells.
  • Established a system for controlled priming of inflammasome activation.
  • Employed real-time imaging of apoptosis-associated speck-like protein containing a CARD (ASC) speck formation.
  • Developed strategies for assessing the impact of test substances on inflammasome activation.

Main Results:

  • Successfully generated fluorescent inflammasome indicator microglia (BV-2) cells.
  • Established a reliable method for real-time monitoring of ASC speck formation.
  • Demonstrated the utility of these cells for examining modulations in inflammasome activation.

Conclusions:

  • The developed protocol provides an effective approach for studying microglia inflammasome activation.
  • This method is suitable for screening chemicals and pharmacological agents.
  • Offers a valuable tool for understanding neuroinflammation and developing therapeutics.