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TRIM47 promotes ovarian cancer cell proliferation, migration, and invasion by activating STAT3 signaling

Xi Wang1, Yu Fu2, Yanyan Xing2

  • 1Department of Emergency and Critical Care Medicine, Jinshan Hospital, Fudan University, Shanghai, China.

Clinics (Sao Paulo, Brazil)
|October 26, 2022
PubMed
Abstract

Insights

Tripartite Motif 47 (TRIM47) protein drives ovarian cancer growth by activating STAT3 signaling. Inhibiting TRIM47 suppressed tumor progression, suggesting it as a potential therapeutic target for ovarian cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Tripartite Motif 47 (TRIM47) protein is implicated in various cancers.
  • Understanding TRIM47's role in ovarian cancer is crucial for developing targeted therapies.

Purpose of the Study:

  • To investigate the biological functions of TRIM47 in ovarian cancer.
  • To determine the potential of TRIM47 as a therapeutic target.

Main Methods:

  • TRIM47 was manipulated (knockdown and overexpression) in ovarian cancer cell lines (SKOV3, OVCAR3).
  • Assessed effects on cell proliferation, clone formation, apoptosis, invasion, and migration.
  • Evaluated tumor growth in xenograft mouse models.
  • Analyzed protein and gene expression using western blotting and qRT-PCR.

Main Results:

  • TRIM47 knockdown inhibited ovarian cancer cell proliferation, invasion, migration, and epithelial-mesenchymal transition.
  • TRIM47 knockdown significantly suppressed tumor growth in vivo.
  • TRIM47 knockdown reduced STAT3 phosphorylation and downstream targets (MCL-1, MMP2, c-MYC).
  • STAT3 inhibition partially reversed TRIM47-induced proliferation and invasion.

Conclusions:

  • TRIM47 acts as an oncogene in ovarian cancer by activating STAT3 signaling.
  • TRIM47 represents a potential therapeutic target for ovarian cancer prevention and treatment.
Keywords:
MetastasisOvarian cancerProliferationSTAT3TRIM47

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