Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Changing morphology of Eperythrozoon ovis.

F M Gulland, D L Doxey, G R Scott

    Research in Veterinary Science
    |July 1, 1987
    PubMed
    Summary

    Acridine orange is more reliable than Giemsa stain for identifying Eperythrozoon ovis in sheep, especially during low parasite levels. Parasite morphology changes with increased Eperythrozoon ovis parasitaemia.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Flight muscle and heart phenotypes in the high-flying ruddy shelduck.

    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology·2021
    Same author

    Different strategies for convective O<sub>2</sub> transport in high altitude birds: A graphical analysis.

    Comparative biochemistry and physiology. Part A, Molecular & integrative physiology·2020
    Same author

    Characterizing the influence of chronic hypobaric hypoxia on diaphragmatic myofilament contractile function and phosphorylation in high-altitude deer mice and low-altitude white-footed mice.

    Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology·2019
    Same author

    Molar crenulation trait definition and variation in modern human populations.

    Homo : internationale Zeitschrift fur die vergleichende Forschung am Menschen·2018
    Same author

    Unilateral absence of mandibular condyle in a Bronze Age male skeleton from Portugal.

    International journal of paleopathology·2018
    Same author

    Effects of chronic hypoxia on diaphragm function in deer mice native to high altitude.

    Acta physiologica (Oxford, England)·2018

    Area of Science:

    • Veterinary Parasitology
    • Microbiology
    • Animal Science

    Background:

    • Eperythrozoon ovis is an intraerythrocytic parasite affecting sheep.
    • Accurate identification of E. ovis is crucial for disease management.
    • Staining methods are essential for microscopic parasite detection.

    Purpose of the Study:

    • To compare the reliability of Giemsa stain and acridine orange for identifying Eperythrozoon ovis in sheep.
    • To investigate the effect of parasitaemia levels on parasite morphology.

    Main Methods:

    • Light microscopy was used to examine blood smears from sheep infected with Eperythrozoon ovis.
    • Giemsa stain and acridine orange staining techniques were applied.
    • Parasitaemia levels were assessed and correlated with morphological observations.

    Main Results:

    • Acridine orange demonstrated higher reliability than Giemsa stain for E. ovis identification, particularly at low parasitaemias.
    • The morphology of Eperythrozoon ovis exhibited alterations correlating with increasing levels of parasitaemia.

    Conclusions:

    • Acridine orange is recommended as a superior staining method for diagnosing Eperythrozoon ovis infections in sheep.
    • Understanding morphological changes related to parasitaemia can aid in diagnosis and disease assessment.

    Related Experiment Videos