The Arabidopsis Receptor-like Kinase CAP1 Promotes Shoot Growth under Ammonium Stress

High levels of ammonium (NH₄⁺) in soils inhibit plant growth and nitrogen utilization efficiency. Elucidating the underlying mechanisms of NH₄⁺ toxicity is essential for alleviating the growth inhibition caused by high NH₄⁺. Our previous work showed that [Ca²⁺]_cyt-associated protein kinase 1 (CAP1) regulates root hair growth in response to NH₄⁺ in Arabidopsis thaliana, and the cap1-1 mutant produces short root hairs under NH₄⁺ stress conditions. However, it is unclear whether CAP1 functions in other physiological processes in response to NH₄⁺. In the present study, we found that CAP1 also plays a role in attenuating NH₄⁺ toxicity to promote shoot growth. The cap1-1 mutant produced smaller shoots with smaller epidermal cells compared with the wild type in response to NH₄⁺ stress. Disruption of CAP1 enhanced the NH₄⁺-mediated inhibition of the expression of cell enlargement-related genes. The cap1-1 mutant showed elevated reactive oxygen species (ROS) levels under NH₄⁺ stress, as well as increased expression of respiratory burst oxidase homologue genes and decreased expression of catalase genes compared with the wild type. Our data reveal that CAP1 attenuates NH₄⁺-induced shoot growth inhibition by promoting cell wall extensibility and ROS homeostasis, thereby highlighting the role of CAP1 in the NH₄⁺ signal transduction pathway.