Recent Advances in CRISPR/Cas-Based Biosensors for Protein Detection

Area of Science:

• Molecular Biology
• Biotechnology
• Biosensing Technology

Background:

• CRISPR/Cas systems, prokaryotic immune mechanisms, are recognized for gene editing and biosensing.
• Traditional CRISPR/Cas biosensors primarily target nucleic acids, overlooking applications for proteins and other analytes.
• Recent advancements highlight CRISPR/Cas biosensors as effective tools for sensitive and specific protein detection.

Purpose of the Study:

• To review and elucidate the mechanisms of CRISPR/Cas-based biosensors for protein detection.
• To categorize existing CRISPR/Cas biosensor strategies for protein analysis.
• To discuss future prospects and challenges in the field of CRISPR/Cas protein detection.

Main Methods:

• Categorization of CRISPR/Cas biosensors based on protein detection mechanisms.
• Review of antibody-assisted CRISPR/Cas-based protein detection methods.
• Review of aptamer-assisted CRISPR/Cas-based protein detection methods.
• Inclusion of miscellaneous CRISPR/Cas-based methods for protein detection.

Main Results:

• CRISPR/Cas biosensors demonstrate significant potential for sensitive and specific protein detection.
• Antibody and aptamer-assisted methods represent key strategies in CRISPR/Cas protein biosensing.
• The review consolidates diverse approaches, providing a comprehensive overview of the field.

Conclusions:

• CRISPR/Cas biosensors represent a significant advancement in protein analysis technology.
• Further research into mechanisms and applications will drive the development of superior protein biosensors.
• Addressing current challenges is crucial for realizing the full potential of CRISPR/Cas-based protein detection.