Detection of Alpha-Fetoprotein Using Aptamer-Based Sensors.

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Biochemistry

Background:

• Alpha-fetoprotein (AFP) is a key protein biomarker for early liver cancer detection.
• Highly sensitive and reliable AFP detection methods are essential for clinical applications.

Purpose of the Study:

• To review aptamer-based biosensors for alpha-fetoprotein (AFP) detection.
• To provide a comprehensive overview of optical and electrochemical detection strategies.

Main Methods:

• Reviewed optical biosensors: Raman spectroscopy, dual-polarization interferometry, resonance light-scattering, fluorescence, and chemiluminescence.
• Reviewed electrochemical biosensors: cyclic voltammetry, electrochemical impedance spectroscopy, and giant magnetic impedance.

Main Results:

• Aptamer-based biosensors offer promising avenues for sensitive AFP detection.
• Both optical and electrochemical approaches present distinct advantages for AFP analysis.

Conclusions:

• Continued development of AFP detection methods is needed, focusing on high sensitivity, stability, low cost, and ease of use.
• Aptamer-based biosensors are vital for advancing early liver cancer diagnostics.