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Labeling DNA Probes03:31

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Nonbiodegradable Spiegelmer-Driven Colorimetric Biosensor for Bisphenol A Detection.

Shuo Ren1, Samuel Cho1, Ruixan Lin2

  • 1PCL Inc., Seoul 05854, Korea.

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Summary

This study introduces a nuclease-resistant spiegelmer aptasensor for detecting bisphenol A (BPA). The developed sensor offers sensitive and selective BPA detection, showing promise for environmental monitoring.

Keywords:
L-DNAzymeaptasensorbisphenol A (BPA)colorimetric assayspiegelmer

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Area of Science:

  • Oligonucleotide chemistry
  • Biosensing
  • Environmental analysis

Background:

  • Natural D-form aptamers are susceptible to nucleases, limiting their use in biological settings.
  • Spiegelmers, enantiomers of D-oligonucleotides, offer enhanced stability.
  • Bisphenol A (BPA) is an environmental contaminant requiring sensitive detection methods.

Purpose of the Study:

  • To develop a non-biodegradable spiegelmer-based platform for sensitive bisphenol A (BPA) detection.
  • To create a nuclease-resistant aptasensor for improved biological stability.
  • To demonstrate a label-free colorimetric assay for BPA detection.

Main Methods:

  • Chemical synthesis of spiegelmers from D-form aptamers.
  • Characterization of spiegelmer-target interactions using biolayer interferometry.
  • Development of a gold nanoparticle-based colorimetric assay for BPA detection.

Main Results:

  • The spiegelmer aptasensor demonstrated high sensitivity and selectivity for BPA detection.
  • The assay achieved a limit of detection (LOD) of 0.057 ng/mL.
  • The aptasensor exhibited a wide dynamic range (10^5) and stability in biological fluids.

Conclusions:

  • A nuclease-resistant spiegelmer aptasensor was successfully developed for BPA detection.
  • The developed aptasensor shows significant potential for in-field applications like water quality monitoring.
  • This approach offers a stable and sensitive method for detecting environmental contaminants.