A High-Performance Self-Supporting Electrochemical Biosensor to Detect Aflatoxin B1.

Area of Science:

• Electrochemistry
• Biosensor Technology
• Food Safety Analysis

Background:

• High-performance electrochemical biosensors are crucial for rapid aflatoxin B1 (AFB1) detection in the food industry.
• Existing methods may lack the sensitivity or speed required for effective food safety monitoring.

Purpose of the Study:

• To develop a multi-scaled electrochemical biosensor for sensitive and rapid detection of AFB1.
• To investigate the synergistic effects of novel components for enhanced biosensor performance.

Main Methods:

• Fabrication of a free-standing carbon nanofiber/carbon felt support.
• Assembly of carboxylated polystyrene nanospheres, aptamer, and horseradish peroxidase.
• Utilized differential pulse voltammetry for quantitative AFB1 detection.

Main Results:

• The biosensor demonstrated exceptional performance with a low limit of detection (0.016 pg mL-1).
• Achieved high recovery rates (87.53%–106.71%) in wine and soy sauce samples.
• Exhibited excellent sensitivity, reproducibility, specificity, and stability.

Conclusions:

• The developed multi-scaled electrochemical biosensor offers a high-performance solution for AFB1 detection.
• The synthetic strategy provides a new route for fabricating advanced electrochemical biosensors for mycotoxin analysis.