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Updated: Aug 23, 2025
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Assessment of Vascular Regeneration in the CNS Using the Mouse Retina
Published on: June 23, 2014
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Hematopoietic Cells Influence Vascular Development in the Retina
Bright Asare-Bediako1,2, Yvonne Adu-Agyeiwaah1,2, Antonio Abad3,4,5
1Vision Science Graduate Program, School of Optometry, University of Alabama at Birmingham, Birmingham, AL 35233, USA.
Cells
|October 27, 2022
Summary
Hematopoietic cells, including microglia and monocytes, are vital for developing retinal vasculature. Their presence from birth influences blood vessel formation and retinal health.
Area of Science:
- Ophthalmology
- Developmental Biology
- Immunology
Background:
- Hematopoietic cells are crucial in adult retinal health and disease.
- Their role in retinal development and the phenotype of recruited cells remain understudied.
Purpose of the Study:
- To investigate the temporal changes and phenotype of hematopoietic cells during retinal development.
- To understand the role of hematopoietic cells in retinal vasculogenesis.
Main Methods:
- Utilized the hematopoietic cell-specific protein Vav1 for tracking.
- Employed flow cytometry and immunohistochemistry to analyze cell populations and localization.
- Studied the effects of Vav1 loss and oxygen-induced retinopathy on retinal development.
Main Results:
- Hematopoietic cells, including microglia, monocytes, and myeloid angiogenic cells (MACs), are present in the retina from postnatal day 0 (P0).
- These cells localize to the inner retina before vascularization, forming networks associated with developing vasculature.
- Loss of Vav1 resulted in reduced vessel density and increased astrocyte inflammation.
- Hematopoietic cells closely associated with vasculature during oxygen-induced retinopathy, aiding neovascularization.
Conclusions:
- Hematopoietic cells play a significant, underappreciated role in retinal vasculogenesis and the formation of a healthy retina.
- These cells are integral to the development and maintenance of retinal vasculature throughout life.
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