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Hybridization in situ of Salivary Glands, Ovaries, and Embryos of Vector Mosquitoes
Published on: June 28, 2012
Failure of Onchocerca gutturosa to develop in Simulium soubrense and Simulium yahense from Liberia
Abstract:
Laboratory reared female S. soubrense and S. yahense from Liberia were intrathoracically inoculated with bovine skin dwelling microfilariae of Onchocerca gutturosa. Of a total of 174 flies, each injected with 50 fresh microfilariae, the maximum development was to late first-stage larvae. One S. soubrense also contained two second-stage larvae and a maldeveloped third-stage larvae. No development to normal third-stage larvae was observed in any fly despite maintenance at 28 degrees C for up to 10 days following infection. This contrasts with complete development of O. volvulus in both blackfly species under similar conditions.
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