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The Stability and Anti-Angiogenic Properties of Titanium Dioxide Nanoparticles (TiO2NPs) Using Caco-2 Cells
Oladipupo Moyinoluwa David1, Kim Leigh Lategan1, Maria Fidalgo de Cortalezzi2
1Department of Medical Bioscience, University of the Western Cape, Cape Town 7535, South Africa.
Biomolecules
|October 27, 2022
Summary
Titanium dioxide nanoparticles (TiO2NPs) in consumer products may alter in size in different environments. TiO2NPs induced cell stress but not inflammation, showing potential anti-angiogenic effects that warrant further study.
Area of Science:
- Nanotechnology
- Materials Science
- Toxicology
Background:
- Titanium dioxide nanoparticles (TiO2NPs) are widely used in sunscreens, paints, toothpaste, and cosmetics.
- Their prevalence raises concerns about human and environmental exposure and associated toxicity.
- Conflicting toxicity data necessitates risk assessment for TiO2NPs produced via different methods.
Purpose of the Study:
- To evaluate the impact of various media on TiO2NP properties (hydrodynamic size, zeta potential).
- To investigate TiO2NP effects on Caco-2 cell viability, inflammation, and cell stress biomarkers.
- To analyze TiO2NP influence on angiogenesis proteome profiles.
Main Methods:
- Characterization of TiO2NP properties (size, zeta potential) in different media.
- Exposure of Caco-2 cells to TiO2NPs.
- Assessment of cell viability, inflammatory biomarkers (ELISA), cell stress biomarkers (Western blot), and angiogenesis proteome (mass spectrometry).
Main Results:
- TiO2NPs showed an increase in size over time in tested media, with stable zeta potentials.
- TiO2NPs induced cell stress biomarkers but were not cytotoxic.
- No significant effects on Caco-2 inflammatory biomarkers were observed.
- TiO2NPs exhibited anti-angiogenic properties at the no-observed-adverse-effect level.
Conclusions:
- Environmental media affect TiO2NP physical properties.
- TiO2NPs induce cellular stress without cytotoxicity or inflammation in Caco-2 cells.
- Potential anti-angiogenic effects of TiO2NPs require further investigation.
