Correlation between Sperm Micro Ribonucleic Acid-34b and -34c Levels and Clinical Outcomes of Intracytoplasmic Sperm...

Area of Science:

• Reproductive Biology
• Genetics
• Infertility Research

Background:

• Intracytoplasmic sperm injection (ICSI) is a key assisted reproductive technology.
• The role of sperm microRNAs (miRNAs) in ICSI outcomes remains underexplored.
• Male factor infertility, including teratozoospermia and asthenozoospermia, affects ICSI success rates.

Purpose of the Study:

• To investigate the association between specific sperm miRNA levels (miR-34b, miR-34c, miR-122, miR-429) and ICSI outcomes.
• To evaluate the predictive value of these miRNAs in men with teratozoospermia and asthenozoospermia.

Main Methods:

• Quantitative real-time PCR (TaqMan) was used to measure sperm miRNA expression.
• Relative miRNA levels were quantified using a comparative method.
• Receiver operating characteristic (ROC) curve analysis was performed to determine optimal cut-off values.

Main Results:

• miR-34b and miR-34c levels were significantly higher in the live birth group among men with teratozoospermia.
• These miRNAs were not associated with fertilization or early embryo development rates.
• Elevated miR-34b and miR-34c levels above identified thresholds correlated with higher implantation, pregnancy, and live birth rates.

Conclusions:

• Sperm miR-34b and miR-34c are significantly associated with ICSI clinical outcomes, particularly in teratozoospermia.
• These miRNAs may serve as potential predictive biomarkers for male factor infertility.
• Further validation is needed for clinical application as a reference indicator.