Related Experiment Video
Updated: Aug 23, 2025

Employing Pressurized Hot Water Extraction PHWE to Explore Natural Products Chemistry in the Undergraduate Laboratory
Published on: November 7, 2018
Physicochemical Characterization and Prospecting Biological Activity of Some Authentic Transylvanian Essential Oils:
Dan Vârban1, Marius Zăhan2, Carmen Rodica Pop3
1Faculty of Agriculture, University of Agricultural Sciences and Veterinary Medicine of Cluj-Napoca, Calea Mănăștur Street No. 3-5, 400372 Cluj-Napoca, Romania.
Abstract:
Essential oils are a category of agro-based industrial products experiencing increasing demand. In this research, three essential oils obtained by steam distillation from lavender, sage and basil plants cultivated in temperate continental conditions of Transylvania were investigated for chemical composition, physical characteristics and biological activity (antimicrobial and cytotoxic effect on cancer cell lines). The number of identified compounds varied: 38 for lavender, 29 for sage essential oil and 41 for basil. The volatile profile was dominated by terpenes and terpenoids (>80%). Major components were beta-linalool and linalool acetate in lavender essential oil; thujones and camphor in sage essential oil; beta-linalool, thujone, camphor and eucalyptol in basil essential oil. Refractive index of the essential oils was lowest for lavender and highest for sage. Antibacterial activity was strongest for basil, moderate for lavender and weakest for sage essential oil. The most active on both colon adenocarcinoma (Caco-2) and ovary carcinoma (A2780) was sage essential oil.

