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Related Experiment Video

Updated: Aug 23, 2025

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
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A Sensitive and Portable Double-Layer Microfluidic Biochip for Harmful Algae Detection.

Ping Li1, Le Qiang1,2,3, Yingkuan Han1

  • 1Institute of Marine Science and Technology, Shandong University, Qingdao 266000, China.

Micromachines
|October 27, 2022
PubMed
Summary
This summary is machine-generated.

A new microfluidic biochip enables rapid, sensitive detection of multiple harmful algal bloom (HAB) species. This portable device offers a fast and accurate method for marine environmental monitoring and disaster warning systems.

Keywords:
environmental warningharmful algal bloommicrofluidic biochipnuclei acids sensingphotoluminescence detection

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Area of Science:

  • Environmental Science
  • Biotechnology
  • Analytical Chemistry

Background:

  • Harmful algal blooms (HABs) pose significant ecological threats to coastal waters.
  • Effective monitoring is crucial for natural disaster warning and environmental management.
  • Current methods struggle with rapid, sensitive detection of multiple algae species.

Purpose of the Study:

  • To develop an ultrasensitive, rapid, and portable biochip for simultaneous quantitative detection of six algae species.
  • To utilize specific DNA probes and graphene oxide nanosheets for enhanced fluorescence detection.
  • To provide a feasible solution for real-time marine environmental monitoring.

Main Methods:

  • Design of a double-layer microfluidic biochip.
  • Development of specific DNA probes targeting 18S ribosomal DNA (18S rDNA) gene fragments of HABs.
  • Labeling probes with cyanine-3 (Cy3) and utilizing graphene oxide (GO) nanosheets for photoluminescence signal transfer.
  • Integration with a portable detection system for rapid analysis.

Main Results:

  • Simultaneous quantitative detection of six algae species achieved within 45 minutes.
  • Ultrasensitive detection with a linear range of fluorescence recovery from 0.1 fM to 100 nM.
  • Exceptional detection limit reaching 108 aM.
  • Demonstrated high detection performance and a simple detection process.

Conclusions:

  • The developed microfluidic biochip offers a highly sensitive, rapid, and portable solution for HAB detection.
  • The approach is feasible for accurate detection using matched portable equipment.
  • This technology holds significant promise for marine natural disaster monitoring and environmental protection efforts.