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NFκB1 Polymorphisms Are Associated with Severe Influenza A (H1N1) Virus Infection in a Canadian Population

Suhrobjon Mullo Mirzo1, Anand Kumar2,3, Naresh Kumar Sharma2

  • 1Max Rady College of Medicine, University of Manitoba, Winnipeg, MB R3X 0P3, Canada.

Microorganisms
|October 27, 2022
PubMed

Insights

Genetic variations in the NFκB1 gene are linked to severe outcomes in Canadian patients with pandemic influenza A (H1N1). Specific NFκB1 gene polymorphisms may affect how individuals respond to H1N1 infection.

Area of Science:

  • Genetics
  • Immunology
  • Virology

Background:

  • The 2009 pandemic influenza A (H1N1) caused significant global health challenges.
  • Understanding host genetic factors influencing H1N1 infection severity is crucial for public health.
  • The NFκB1 gene plays a role in immune responses and inflammation.

Purpose of the Study:

  • To investigate the association between NFκB1 gene polymorphisms and clinical outcomes in Canadian H1N1 patients.
  • To identify specific genetic variations within NFκB1 that correlate with severe H1N1 infection.

Main Methods:

  • Genotyping of the NFκB1 gene in 36 Caucasian ICU patients from the 2009 H1N1 pandemic.
  • Utilized next-generation sequencing (Roche 454) for SNP discovery.
  • Compared SNP frequencies with the 1000 Genomes Project British Caucasian population.

Main Results:

  • Identified 136 single nucleotide polymorphisms (SNPs) in the NFκB1 gene.
  • Found 63 SNPs significantly enriched in ICU patients compared to controls (p < 0.05).
  • Enriched SNPs were predominantly intronic, with two non-transcribing exonic variants.

Conclusions:

  • Genetic variations within the NFκB1 gene are associated with clinical outcomes of pandemic H1N1 infection.
  • Sequence variations in NFκB1 may influence an individual's immune response and susceptibility to severe influenza.
  • These findings highlight NFκB1 as a potential genetic marker for H1N1 infection severity.

Keywords:
H1N1NFκB1SNPinfluenzasevere

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