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Effect of Polydextrose on the Growth of Pediococcus pentosaceus as Well as Lactic Acid and Bacteriocin-like

Maria Carolina Wanderley Porto1, Pamela Oliveira de Souza de Azevedo1, Felipe Rebello Lourenço2

  • 1Department of Biochemical and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences, University of São Paulo, Av. Lineu Prestes 580, São Paulo 05508900, Brazil.

Microorganisms
|October 27, 2022
PubMed

Insights

Polydextrose supplementation enhances Pediococcus pentosaceus growth and lactic acid production. Optimal conditions were identified for maximizing cell concentration, biomass yield, and bacteriocin-like inhibitory substance (BLIS) production, showing promise for food industry applications.

Area of Science:

  • Microbiology
  • Food Science
  • Biotechnology

Background:

  • Pediococcus pentosaceus is a lactic acid bacterium with potential antimicrobial properties.
  • Bacteriocin-like inhibitory substances (BLIS) are antimicrobial compounds produced by microorganisms.
  • Polydextrose is a prebiotic fiber that can influence microbial growth and metabolism.

Purpose of the Study:

  • To investigate the effects of polydextrose on Pediococcus pentosaceus growth, lactic acid, and BLIS production.
  • To determine optimal cultivation conditions (pH, temperature, rotational speed, polydextrose concentration) for Pediococcus pentosaceus.
  • To evaluate the antibacterial activity of produced BLIS against target pathogens.

Main Methods:

  • Cultivation of Pediococcus pentosaceus in MRS medium with varying polydextrose concentrations.
  • Optimization of fermentation parameters including pH, temperature, and rotational speed.
  • Measurement of cell concentration, biomass yield, substrate consumption, lactic acid production, and BLIS activity.
  • Statistical analysis of experimental data.

Main Results:

  • Maximum cell concentration (10.24 gX L-1) and productivity (0.42 gX L-1 h-1) were achieved at pH 6.0, 35 °C, 150 rpm with 1.5% polydextrose.
  • Highest specific growth rate (0.99 h-1) and biomass yield (2.96 gX gS-1) occurred at pH 6.0, 25 °C, 50 rpm with 1.5% polydextrose.
  • Maximized specific substrate consumption rate (0.09 gS gX-1 h-1) and lactic acid productivity (0.44 gP L-1 h-1) were observed at pH 6.0, 35 °C, 50 rpm with 0.5% polydextrose.
  • BLIS exhibited potent antibacterial activity against Escherichia coli and Enterococcus faecium.

Conclusions:

  • Polydextrose significantly influences Pediococcus pentosaceus fermentation performance.
  • Specific conditions can be tailored to optimize either cell biomass or lactic acid and BLIS production.
  • The study highlights the potential of Pediococcus pentosaceus-derived BLIS as a natural antibacterial agent for the food industry.
Keywords:
Pediococcus pentosaceus ATCC 43200bacteriocin-like inhibitory substancepathogens microorganismspolydextrose

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