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Storage-induced changes in human newborn red cells
Insights
Newborn red blood cells show accelerated storage changes compared to adult cells, potentially due to faster adenosine triphosphate (ATP) decline. However, storage in CPDA-1 is better than CPD, and adenosine can partially reverse damage, suggesting transfusion potential.
Area of Science:
- Transfusion Medicine
- Neonatal Hematology
- Blood Storage
Background:
- Fetal red blood cells (RBCs) are adapted for intrauterine environments.
- Limited understanding exists regarding fetal RBC response to postnatal storage conditions.
- Neonatal RBCs may exhibit unique metabolic and membrane alterations during storage.
Purpose of the Study:
- To investigate metabolic and membrane changes in newborn RBCs during storage.
- To compare storage responses in citrate-phosphate-dextrose (CPD) and CPDA-1 media.
- To assess the potential for transfusion of stored neonatal RBCs.
Main Methods:
- Storage of newborn RBCs in CPD and CPDA-1 media.
- Analysis of metabolic changes, including adenosine triphosphate (ATP) levels.
- Evaluation of membrane integrity and storage lesion development.
- Assessment of storage lesion reversibility with adenosine incubation.
Main Results:
- Newborn RBCs demonstrated accelerated storage-induced changes compared to adult RBCs.
- Rapid decline in ATP levels was observed in neonatal RBCs during storage.
- CPDA-1 provided better preservation for fetal RBCs than CPD.
- Storage lesion was partially reversible with adenosine treatment, similar to adult RBCs.
Conclusions:
- Neonatal RBCs are more susceptible to storage-related damage than adult RBCs.
- Accelerated changes may be linked to faster ATP depletion in fetal cells.
- Stored neonatal RBCs, particularly in CPDA-1 or improved media, may be suitable for transfusion after several weeks.
Abstract:
Fetal red cells are well suited for intrauterine life; however, little is known about their response to postnatal environments. The purpose of this work was to investigate the metabolic and membrane changes affecting newborn red cells during their exposure to storage in citrate-phosphate-dextrose (CPD) and citrate-phosphate-dextrose-adenine (CPDA-1). The findings suggest that newborn red cells are affected more by storage than are adult cells. These accelerated storage changes in the red cells of newborns may be related indirectly to the rapid adenosine triphosphate (ATP) decline. As is the case with adult red cells, fetal cells withstand storage in CPDA-1 better than in CPD. The storage lesion in these cells was partly reversible, as in adult cells, by incubation with adenosine. It was therefore concluded that newborn red cells obtained from placentas and stored for several weeks in CPD or CPDA-1 media or other media that improve the metabolic profile of these cells may be acceptable for transfusion.