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Updated: Aug 23, 2025

Generation and Multi-phenotypic High-content Screening of Coxiella burnetii Transposon Mutants
Published on: May 13, 2015
Genetic Diversity of Coxiella burnetii in Iran by Multi-Spacer Sequence Typing
Ashraf Mohabati Mobarez1, Neda Baseri2,3, Mohammad Khalili4
1Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran 1411713116, Iran.
Abstract:
Coxiella burnetii, the zoonotic agent of Q fever, has a worldwide distribution including Iran. However, no information regarding the circulating genotype of this infection has been reported in Iran. This study aimed to evaluate the genetic diversity of C. burnetii in Iran using the multi-spacer sequence typing (MST) method. First, 14 positive C. burnetii samples (collected from four sheep, three goats, and seven cattle) were confirmed using quantitative polymerase chain reaction (qPCR) targeting the IS1111 gene. Then, ten spacers (Cox 2, 5, 18, 20, 22, 37, 51, 56, 57, and 61) were amplified using PCR for future MST analysis. The in-silico MST genotyping analysis of domestic ruminant samples revealed two new alleles (Cox5.11 and Cox56.15) in Cox5 and Cox56 loci that led to the emergence of four novel MST genotypes (MST62, 63, 64, and 65) and one MST genotype that has been previously described (MST61). This study showed the circulation of five MST C. burnetii genotypes among Iranian domestic ruminants. Understanding the C. burnetii genotypic profiles is critical in determining and preventing Q fever outbreaks.
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