Supercritical CO₂ Impregnation of Thymol in Thermoplastic Starch-Based Blends: Chemico-Physical Properties and...

The aim of the present study was to investigate starch-based materials, prepared in an environmentally friendly way and from renewable resources, suitable for the development of biodegradable active food packaging. For this purpose, a bioactive compound (thymol) was incorporated into thermoplastic starch (TPS) and a TPS blend with poly (ε-caprolactone) (TPS-PCL) by the supercritical CO₂ (scCO₂) impregnation process. Impregnation experiments with scCO₂ were carried out at a pressure of 30 MPa and temperatures in the range of 40-100 °C during 1 to 20 h. The structural, morphological, and thermal properties of the obtained materials were comprehensively evaluated. Bioactive component release kinetic studies were performed in water at 6 °C and 25 °C. It was shown that the scCO₂ impregnation process could be successfully employed for thymol loading into TPS and TPS-PCL. The process was significantly influenced by the operating temperature and time as well as content of PCL. The samples showed a controlled release of thymol within seven days with a higher amount of released thymol from the TPS-PCL blend. The obtained materials are solvent-free and release the bioactive component in a controlled manner.