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Local aberration control to improve efficiency in multiphoton holographic projections
Optics Express
|October 27, 2022
Summary
This study presents a sensorless adaptive optics method to correct optical aberrations in turbid media for multiphoton applications. The technique significantly enhances excitation efficiency in imaging and optogenetics by improving light pattern intensity.
Area of Science:
- Optics and Photonics
- Biomedical Imaging
- Neuroscience Tools
Background:
- Optical aberrations degrade light quality in turbid media with inhomogeneous refractive indices.
- Aberrations non-linearly reduce excitation efficiency in multiphoton optical applications like fluorescence imaging and optogenetics.
Purpose of the Study:
- To demonstrate a sensorless adaptive optics (AO) technique for compensating optical aberrations in turbid media.
- To correct local (anisoplanatic) distortions using custom holographic patterns projected by a spatial light modulator (SLM).
Main Methods:
- Developed and applied a sensorless adaptive optics system utilizing a spatial light modulator.
- Projected custom three-dimensional holographic patterns to compensate for aberrations.
- Tested the method on synthetic and biological samples with varying inhomogeneities.
Main Results:
- Successfully counteracted aberrations caused by optical system misalignment and sample inhomogeneities.
- Achieved at least a two-fold improvement in the intensity of the stimulation pattern.
- Demonstrated effective anisoplanatic correction in turbid environments.
Conclusions:
- Sensorless adaptive optics is effective for aberration correction in turbid media.
- The holographic AO method enhances excitation efficiency in multiphoton applications.
- This technique offers improved performance for optical imaging and optogenetics in scattering samples.
