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    Area of Science:

    • Microscopy and Imaging Science
    • Optical Engineering
    • Computational Imaging

    Background:

    • Microscope performance is limited by objective optics and detector bandwidth.
    • Conventional Fourier ptychography struggles with multiple objectives due to phase coherence requirements.

    Purpose of the Study:

    • To develop a scalable synthetic-aperture microscopy technique for rapid, high-resolution imaging.
    • To overcome limitations of conventional Fourier ptychography for multi-objective systems.

    Main Methods:

    • Utilized a spherical array of low-power objectives and Fourier ptychography.
    • Synthesized illumination and image-space numerical apertures.
    • Employed ptychographic reconstruction with angular illumination to achieve phase coherence.

    Main Results:

    • Demonstrated a nine-objective microscope achieving 89-megapixel, 1.1 µm resolution images.
    • Reduced image acquisition time by nine times compared to single-objective Fourier ptychography.
    • Enabled low-cost 3D-printed components for longitudinal biological imaging.

    Conclusions:

    • The synthetic-aperture technique offers a scalable route to high-speed, gigapixel microscopy.
    • Facilitates imaging of large cell populations and capturing rare dynamic events.
    • Paves the way for advanced biological sample analysis at multiple scales.