Assessing the Contact Angle Between Dentin Treated With Irrigation and Calcium Hydroxide and Root Canal Sealers

Background The long-term use of calcium hydroxide, or Ca(OH)2, on dentin has been established in the literature. However, scarce data is available on dentin wettability with Ca(OH)2. Aim The present study was conducted to assess the outcomes of Ca(OH)2 use on the wettability of dentin following two and four weeks of using sealers of bioceramic (BC) and Tubli-Seal™ in the root canal. Methods In this study, 168 specimens were divided into 12 groups of 14 specimens each and were numbered from G1 to G12. G1-G6 groups were tested with Tubli-Seal. Sterile water irrigation was reserved for G1 for two weeks (14 days) followed by two minutes treatment with 6% sodium hypochlorite (NaOCl) chemical irrigation and 17% EDTA (10 ml). G2 and G3 were subjected to Ca(OH)2 (0.1 ml of UltraCal) for two weeks followed by 10ml sterile water irrigation for two minutes with chemical irrigation. G4 to G6 were given similar treatment for four weeks. G7-G12 were assessed for BC sealer similarly to G1-G6. Two and four weeks of incubation with sterile water or Ca(OH)2 was done in 100% humidity at 37°C. SEM and EDX were done to evaluate the surface morphology of G1 and G6, and results were formed.  Results Significantly smaller contact angles were seen for Tubli-Seal (G1 to G6) compared to BC (G7 to G12) with p<0.05. Application of water irrigants and Ca(OH)2 (in G2, G5, and G11) showed a smaller (p<0.05, statistically significant) contact angle compared to the use of chemical irrigation agents and Ca(OH)2 (in G3, G6, and G12) except for G8 and G9. Based on EDX and SEM, compared to the use of chemical irrigation with Ca(OH)2, higher remnants of Ca(OH)2 were seen in the water irrigation, whereas, no remnant was seen with chemical irrigants.  Conclusion Better dentin wettability is seen with Tubli-seal compared to BC sealer. A smaller contact angle between root canal sealers and dentin is seen in the remaining calcium hydroxide samples. Also, calcium hydroxide can be removed from the polished dentin surface with two minutes of irrigation with 17% EDTA and 6% NaOCl.