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Nuclear Magnetic Resonance Spectroscopy for the Identification of Multiple Phosphorylations of Intrinsically Disordered Proteins
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Terahertz tyrosine modes.

T J Sanders1, J L Allen1, R Plathe2

  • 1Institute for Superconducting and Electronic Materials and School of Physics, University of Wollongong, NSW 2522, Australia.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|October 27, 2022
PubMed
Summary
This summary is machine-generated.

This study precisely measured the terahertz spectrum of l-tyrosine across various temperatures. Researchers identified definitive low-frequency modes at absolute zero, suggesting some prior spectral features may be experimental artifacts.

Keywords:
4-hydroxy phenylalanineAmino acidBose–EinsteinRedshiftTHzTerahertzTynchrotronTyrTyrosineYl-tyrosine

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Area of Science:

  • Solid-state physics
  • Molecular spectroscopy
  • Biophysics

Background:

  • L-tyrosine is an essential amino acid with a complex terahertz spectrum.
  • Previous terahertz spectroscopy studies on l-tyrosine have reported conflicting low-frequency modes.
  • Understanding these modes is crucial for applications in materials science and drug development.

Purpose of the Study:

  • To accurately determine the low-frequency terahertz modes of pure l-tyrosine at absolute zero.
  • To resolve discrepancies in previously reported spectral features.
  • To establish a reliable spectral fingerprint for l-tyrosine.

Main Methods:

  • Terahertz time-domain spectroscopy (THz-TDS) was employed.
  • Measurements were conducted on pure l-tyrosine samples at nineteen temperatures from 6 K to 300 K.
  • Synchrotron radiation was utilized as the terahertz source for high signal-to-noise ratio.
  • Bose-Einstein statistical mechanics model was applied to analyze temperature-dependent mode behavior.

Main Results:

  • Precise determination of four unequivocal low-frequency modes of l-tyrosine at absolute zero: 1.02 ± 0.01 THz, 1.61 ± 0.01 THz, 1.97 ± 0.01 THz, and 2.19 ± 0.01 THz.
  • The determined modes align with the more reliable previous experimental data.
  • Observed discrepancies with some recent reports suggest potential experimental artifacts in those studies.

Conclusions:

  • The study provides a definitive set of low-frequency modes for l-tyrosine at absolute zero.
  • The findings help to clarify the intrinsic terahertz spectral properties of l-tyrosine.
  • This research contributes to a more accurate understanding and application of l-tyrosine spectroscopy.