Related Experiment Video
Updated: Aug 23, 2025
08:39
Author Spotlight: Polysome Profiling Protocol for Studying Translational Regulation in Arabidopsis Under Heat Stress
Published on: October 11, 2024
1.7K
Transcriptomic stability or lability explains sensitivity to climate stressors in coralline algae.
Tessa M Page1,2,3,4, Carmel McDougall5,6,7, Ido Bar5,8
1Griffth University School of Environment and Science Nathan Campus, Griffith University, Nathan, QLD, Australia. t.page@soton.ac.uk.
BMC Genomics
|October 28, 2022
Summary
Crustose coralline algae show varied responses to ocean warming and acidification. Sporolithon durum is resistant, while Porolithon onkodes is sensitive, highlighting species-specific climate change adaptation in marine ecosystems.
Area of Science:
- Marine Biology
- Ecology
- Climate Change Science
Background:
- Crustose coralline algae (CCA) are vital reef-building organisms.
- CCA provide reef framework stability and settlement cues for invertebrates.
- Species-specific responses to ocean warming (OW) and ocean acidification (OA) are documented.
Purpose of the Study:
- Investigate differential gene expression in CCA under OW and OA.
- Compare the physiological and molecular responses of two CCA species.
- Assess the resilience of CCA to future ocean conditions.
Main Methods:
- Exposed Sporolithon durum and Porolithon onkodes to simulated OW and OA conditions.
- Measured metabolic performance and photosynthetic rates.
- Analyzed transcriptomic changes using differential gene expression.
Main Results:
- Sporolithon durum showed low sensitivity with minimal metabolic and gene expression changes.
- Porolithon onkodes exhibited reduced photosynthesis and over 400 differentially expressed genes.
- Key metabolic pathways, including the pentose phosphate pathway, were implicated in P. onkodes' stress response.
Conclusions:
- Sporolithon durum is more resistant to OW and OA than Porolithon onkodes.
- Porolithon onkodes displays high sensitivity and potentially limited acclimatization ability.
- Gene expression analysis aids in predicting CCA persistence in future oceans.
Related Concept Videos
01:10Transcription
147.6K
Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
147.6K
01:53RNA Stability
33.8K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
33.8K
02:51mRNA Stability and Gene Expression
2.9K
2.9K
02:45Responses to Heat and Cold Stress
13.7K
Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.
13.7K
01:29Translational Regulation
75
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
75
01:23Stringent Response in E. coli
40
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...
40