Preparation, physicochemical properties and antimicrobial activity of chitosan from fly pupae

As an alternative chitosan source, edible insects have been proposed as an unconventional but viable option. Taking fly pupae as an example, this work performed chitosan extraction through a traditional chemical method with some modifications, and investigated its physicochemical properties and antimicrobial activity. The results showed that adding 0.5% sodium sulfite (Na₂SO₃, w/w, Na₂SO₃/fly pupae) synergized with sodium hydroxide (NaOH) for deproteinization was more effective than lye alone. Acid leaching was applied for desalination, and the optimal concentration of hydrochloric acid (HCl) was determined as 2 mol/L by ash content. For decoloration, the optimal decolorization oxidant was sodium hypochlorite (NaClO) with a concentration of 1.0%. For the deacetylation of chitin to chitosan, both the yield and degree of deacetylation (DD) using segmented treatment with alkali-NaOH were higher than those of traditional one-time deacetylation. The established physicochemical properties corresponded with the typical characteristics of chitosan. The determination of antimicrobial activity of chitosan by the turbidimetric method showed that chitosan exhibited notable activity in the order of Staphylococcus aureus > Escherichia coli > Saccharomyces cerevisiae, and this effect decreased with the increase in viscosity-average molecular weight (Mη). These results proved the viability of our improved method for the preparation of chitosan, a valuable antimicrobial agent, using an alternative natural source.