Altered shape and size of red blood cells in obese hyperglycaemic mice

Red blood cells (RBCs) from hyperglycaemic ob/ob-mice, normoglycaemic controls, and a healthy man were sucked into a narrow capillary, photographed and measured. Mouse RBCs had a smaller diameter than human ones. Although of normal diameter, the ob/ob-mouse RBCs exhibited increased area and volume, and more frequently than control RBCs had a minimum cylindrical diameter greater that 3 micron. Cross-sectional profiles with minimum bending resistance were computed for RBCs of mean area and volume. The diameters of these theoretical profiles agreed closely with those empirically observed, in both mice and man. The profile of ob/ob-mouse RBCs predicted a greater resistance to corpuscle bending than the control profile. It is concluded that changes in shape and size explain the decreased filtrability of diabetic ob/ob-mouse RBCs. The results also suggest that the actually occuring smooth biconcavity is in general the RBC shape with maximum flexibility.