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Updated: Aug 23, 2025

Quantifying Synapses: an Immunocytochemistry-based Assay to Quantify Synapse Number
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Optimal puncture position for ICSI can be detected by image analysis using Local Binary Pattern.

Takashi Morimoto1, Tomohiro Maekawa1, Shimpei Mizuta2

  • 1Department of Reproductive Medicine, Reproduction Clinic Tokyo, Shiodome City Center 3F, 1-5-2 Higashi-shimbashi, Minato-ku, Tokyo 105-7103, Japan.

Reproductive Biomedicine Online
|October 28, 2022
PubMed
Summary

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This summary is machine-generated.

The ICSI Position Detector (IPD) helps identify optimal puncture sites during intracytoplasmic sperm injection (ICSI), significantly reducing oocyte degeneration and improving fertilization rates for better embryo development.

Area of Science:

  • Reproductive Medicine
  • Embryology
  • Assisted Reproductive Technologies

Background:

  • Unintentional membrane rupture (UMR) during intracytoplasmic sperm injection (ICSI) can lead to oocyte degeneration.
  • Identifying the optimal puncture site is crucial for successful ICSI outcomes.

Purpose of the Study:

  • To evaluate the utility of the ICSI Position Detector (IPD) in identifying the optimal puncture location during ICSI.
  • To determine if using the IPD can prevent UMR and improve oocyte quality and fertilization rates.

Main Methods:

  • A retrospective analysis of 709 mature oocytes undergoing conventional ICSI with IPD imaging.
  • Oocytes were grouped based on IPD analysis into appropriate and non-appropriate puncture sites.
  • Propensity score matching was used to control for potential confounding factors.
Keywords:
ICSIImage analysisOocyte degenerationUnintentional membrane rupture

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Main Results:

  • The appropriate group showed significantly lower UMR (5.3%) and degeneration rates (2.5%) compared to the non-appropriate group (18.2% and 8.7%).
  • Fertilization rates were significantly higher in the appropriate group (87.1%) versus the non-appropriate group (69.7%).
  • These significant differences persisted after propensity score matching.

Conclusions:

  • The ICSI Position Detector (IPD) is effective in identifying optimal puncture locations to prevent UMR during ICSI.
  • Utilizing the IPD reduces oocyte degeneration and increases fertilization rates.
  • This leads to a greater number of viable embryos for transfer or cryopreservation.