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Updated: Aug 23, 2025
Establishment of a Clinically Relevant Ex Vivo Mock Cataract Surgery Model for Investigating Epithelial Wound Repair in a Native Microenvironment
Published on: June 5, 2015
Siep's test: A novel test for checking wound integrity in small-incision cataract surgery
Binita N Gadavi1, Aditi Pai2, M Atul Kamath3
1Department of Ophthalmology, GMERS-Sola, Ahmedabad, Gujarat, India.
Purpose:
This study was conducted to ensure the integrity of surgery wounds.
Methods:
This was a randomized prospective trial of 300 patients who underwent small-incision cataract surgery (SICS) at our hospital over a one-year period. We used 2-3 drops of 5% povidone-iodine at the end of each case, not only to disclose any wound leaks but also to sterilize the surface of the eye.
Results:
Three hundred patients underwent SICS. One hundred ten patients had postoperative wound leak in SICS due to surgical complications. Premature entry comprising 75% (83 cases), followed by floppy iris syndrome comprising 20% (22 cases) and 5% (6 cases) comprising pseudoexfoliation (PXF) syndrome. There was a drop in the number of re-surgeries from 12 to 3 cases since we started implementing this technique.
Conclusion:
The simple procedure is very handy and economical in identifying the leak and can reduce further complications such as endophthalmitis and re-surgeries. Among residents, this can be a boon where povidone-iodine acts as a riverbank for the outflow of aqueous, outlining the speed, location, and quantity of the leak. Siep's test plays an important role in identifying any postoperative wound leaks and also sterilizing the eye surface post surgery.
