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Related Concept Videos

Applications Of NMR In Biology01:25

Applications Of NMR In Biology

3.8K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics  for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
3.8K
2D NMR: Overview of Heteronuclear Correlation Techniques01:18

2D NMR: Overview of Heteronuclear Correlation Techniques

279
Heteronuclear correlation spectroscopy is an analytical technique that investigates the coupling between different types of nuclei, often a proton and an X-nucleus, such as carbon-13 or nitrogen-15. This method is commonly used in nuclear magnetic resonance (NMR) spectroscopy to gain insights into complex chemical compounds' structural and compositional aspects. A typical heteronuclear correlation spectrum displays X-nucleus chemical shifts on one axis and a proton spectrum on the other...
279
¹H NMR Signal Integration: Overview00:58

¹H NMR Signal Integration: Overview

1.7K
The intensity of a signal, which can be represented by the area under the peak, depends on the number of protons contributing to that signal. The area under each peak is shown as a vertical line called an integral, with the integral value listed under it, as seen in the proton NMR spectrum of benzyl acetate. Each integral value is divided by the smallest integral value to obtain the ratio of the number of protons producing each signal. The ratio reveals the relative number of protons and not...
1.7K
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)01:19

2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)

851
Heteronuclear single-quantum correlation spectroscopy (HSQC) is a 2D NMR technique that reveals one-bond correlations between hydrogen and a heteronucleus. The HSQC experiment is similar to the heteronuclear correlation experiment (HETCOR) but is more sensitive. In the HSQC spectrum, the proton chemical shift is plotted on the horizontal F2 axis, while the 13C chemical shift is plotted on the vertical F1 axis. The corresponding proton and 13C spectra are also shown. The HSQC contour plot does...
851
2D NMR: Overview of Homonuclear Correlation Techniques01:16

2D NMR: Overview of Homonuclear Correlation Techniques

268
Homonuclear correlation spectroscopy (COSY) is a powerful technique used in Nuclear Magnetic Resonance (NMR) spectroscopy to study the correlations between nuclei of the same type within a molecule. It provides information about scalar couplings between adjacent nuclei, which helps determine connectivity and structural information. There are several COSY variants, each with its unique strengths and experimental parameters.
COSY90 is the standard two-dimensional (2D) COSY experiment that...
268
Chemical Shift: Internal References and Solvent Effects01:17

Chemical Shift: Internal References and Solvent Effects

730
In an NMR sample, precise measurement of the absolute absorption frequencies of nuclei is difficult. A standard internal reference compound is added, and the frequency difference between the reference signal and sample signals is measured.
The internal reference compound generally used in NMR spectroscopy is tetramethylsilane (TMS). TMS is preferred because it is chemically inert, soluble in NMR solvents, and easily removable. Also, the highly shielded methyl protons in TMS yield an intense...
730

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Related Experiment Video

Updated: Aug 23, 2025

A Strategy for Sensitive, Large Scale Quantitative Metabolomics
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A Strategy for Sensitive, Large Scale Quantitative Metabolomics

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Quantitative NMR Methods in Metabolomics.

G A Nagana Gowda1,2, Daniel Raftery3,4,5

  • 1Northwest Metabolomics Research Center, University of Washington, Seattle, WA, USA. ngowda@uw.edu.

Handbook of Experimental Pharmacology
|October 29, 2022
PubMed
Summary
This summary is machine-generated.

Nuclear Magnetic Resonance (NMR) spectroscopy offers unique advantages in metabolomics, including high reproducibility and definitive metabolite identification. Recent advancements enhance its capabilities for detecting and quantifying a wider range of metabolites.

Keywords:
Fast NMR methodsIsotope taggingMetabolomicsNuclear magnetic resonance (NMR)Quantitation

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Systems Biology

Background:

  • Nuclear Magnetic Resonance (NMR) spectroscopy and Mass Spectrometry (MS) are key metabolomics platforms.
  • NMR offers high reproducibility, non-destructive analysis, definitive unknown metabolite identification, and absolute quantitation.
  • Despite lower sensitivity than MS, NMR's unique features are valuable in complex biological mixture analysis.

Purpose of the Study:

  • To describe quantitative NMR methods in metabolomics.
  • To highlight recent methodological developments in NMR-based metabolomics.
  • To discuss the benefits and challenges of NMR in metabolomics.

Main Methods:

  • Utilizing NMR spectroscopy for metabolite detection and identification.
  • Employing advanced techniques to improve NMR sensitivity and resolution.
  • Focusing on methods for identifying and quantifying unknown and labile metabolites.

Main Results:

  • Technological and methodological advances have improved NMR's resolution, sensitivity, and detection capabilities.
  • Expanded identification and quantitation of metabolites, including labile ones like coenzymes and antioxidants.
  • NMR enables absolute concentration measurements, sometimes without internal standards.

Conclusions:

  • NMR spectroscopy provides unique and valuable data in metabolomics, complementing MS.
  • Ongoing methodological advancements continue to enhance NMR's utility in analyzing complex biological samples.
  • Quantitative NMR methods are crucial for a comprehensive understanding of metabolic profiles.