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DNA barcoding, an effective tool for species identification: a review.

Sandeep Antil1, Jeeva Susan Abraham1, S Sripoorna1

  • 1Acharya Narendra Dev College, University of Delhi, New Delhi, Delhi, India.

Molecular Biology Reports
|October 29, 2022
PubMed
Summary

DNA barcoding uses standardized DNA regions for species identification. Advanced sequencing and data analysis accelerate the creation of comprehensive DNA barcode reference libraries for accurate species discovery.

Keywords:
BiodiversityConsortium for barcode of life (CBOL)DNA barcodingNext-generation sequencing (NGS)Oxford Nanopore Technologies (ONT)’s MinION™PacBio sequencing

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Area of Science:

  • Molecular Biology
  • Taxonomy
  • Bioinformatics

Background:

  • DNA barcoding is a crucial tool for species identification and discovery.
  • It relies on standardized short DNA regions for taxonomic identification.
  • Emerging sequencing technologies enhance accuracy, speed, and reliability.

Purpose of the Study:

  • To analyze proposed DNA barcodes for various organisms.
  • To evaluate the strengths and limitations of DNA barcoding methods.
  • To explore advancements and methods for accelerating reference library construction.

Main Methods:

  • Review of proposed DNA barcode genes (e.g., COI, rbcL, matK, ITS, 16S rRNA).
  • Analysis of Next-generation sequencing (NGS), ONT MinION, and Pac Bio sequencing.
  • Examination of data analysis methods for library construction.

Main Results:

  • DNA barcoding applications span forensics, food safety, and disease research.
  • Consortium for the Barcode of Life (CBOL) identifies universal barcode genes.
  • Advanced sequencing significantly speeds up DNA barcode reference library creation.

Conclusions:

  • High species coverage in reference libraries is key for effective DNA barcoding.
  • Advanced sequencing and data analysis are vital for rapid library development.
  • Accelerated library construction enables efficient species identification and discovery.