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The kingdom Archaeplastida encompasses red and green algae, along with land plants. Unlike other protists with chloroplasts that arose through secondary endosymbiosis, only red and green algae originated from primary endosymbiotic events. This diverse group of eukaryotic organisms contains chlorophyll and performs oxygenic photosynthesis.Algae exist in various forms, from large brown kelp in coastal waters to green scum in puddles and stains on rocks or soil. Some species are responsible for...
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Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...
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An ensemble data assimilation modeling system for operational outdoor microalgae growth forecasting.

Hongxiang Yan1, Mark S Wigmosta1,2, Michael H Huesemann3

  • 1Pacific Northwest National Laboratory, Richland, Washington, USA.

Biotechnology and Bioengineering
|October 29, 2022
PubMed
Summary

Accurate microalgae biomass forecasting is crucial for biofuel production. This study integrates models and data assimilation to improve short-term growth predictions by 60%, aiding operational planning.

Keywords:
Chlorella sorokinianaHuesemann Algae Biomass Growth Modelbiomass forecastingdata assimilationparticle filter

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Area of Science:

  • Biotechnology
  • Algaculture
  • Bioenergy

Background:

  • Microalgae are a promising feedstock for biofuels and biobased products.
  • Accurate forecasting of microalgae growth is essential for efficient pond management and harvesting strategies.

Purpose of the Study:

  • To develop and evaluate an integrated biomass forecasting system for microalgae cultivation.
  • To enhance short-term biomass forecasting accuracy using ensemble data assimilation.

Main Methods:

  • Integration of the Huesemann Algae Biomass Growth Model (BGM) and Modular Aquatic Simulation System in Two Dimensions (MASS2).
  • Application of ensemble data assimilation (DA) with Global Ensemble Forecast System (GEFS) meteorological forecasts.
  • Assimilation of biomass and water temperature measurements to improve model initial conditions.

Main Results:

  • The integrated forecasting system demonstrated an average improvement of 60% in 7-day biomass forecasting skills compared to methods without ensemble DA.
  • Successful pseudo-real-time application in three outdoor Chlorella sorokiniana cultivation ponds.
  • Validation of the system's efficacy in improving short-term biomass prediction accuracy.

Conclusions:

  • The developed BGM-MASS2-DA forecasting system significantly enhances microalgae biomass prediction capabilities.
  • The system shows potential for operational use in guiding pond management and harvesting decisions.
  • Ensemble data assimilation is a key factor in improving the accuracy of microalgae growth forecasts.