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Transcriptome analysis of the inhibitory effect of cycloleucine on myogenesis

Zhijun Wang1, Danfeng Cai1, Kan Li1

  • 1Department of Animal Genetics, Breeding and Reproduction, College of Animal Science, South China Agricultural University, Guangzhou 510642, China; National-Local Joint Engineering Research Center for Livestock Breeding, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, and Key Laboratory of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, Guangzhou 510642, China.

Poultry Science
|October 29, 2022
PubMed

Insights

Cycloleucine inhibits N6-Methyladenosine (m6A) levels in poultry myoblasts, hindering cell proliferation and myotube formation. This study reveals cycloleucine

Area of Science:

  • Molecular Biology
  • Epigenetics
  • Poultry Science

Background:

  • N6-Methyladenosine (m6A) is crucial in biological activities, but its role in poultry myogenesis is understudied.
  • Cycloleucine is a known nucleic acid methylation inhibitor, yet its specific effects on m6A levels and poultry muscle development are unclear.

Purpose of the Study:

  • To investigate the impact of cycloleucine on total N6-Methyladenosine (m6A) levels in poultry myoblasts.
  • To elucidate the molecular mechanisms by which cycloleucine regulates myogenesis.

Main Methods:

  • Treatment of poultry myoblasts with varying concentrations of cycloleucine (10, 20, 30 mM).
  • Differential gene expression analysis to identify affected genes (DEGs).
  • Cell cycle analysis and assessment of myoblast proliferation and myotube formation.

Main Results:

  • Treatment with cycloleucine resulted in 745 differentially expressed genes (DEGs).
  • Low cycloleucine concentrations (10 mM) enriched DEGs in skeletal muscle and satellite cell proliferation/differentiation.
  • Higher concentrations (20, 30 mM) enriched DEGs in metabolic and biosynthetic processes, with observed cell cycle arrest in G1 phase, reduced proliferation, and inhibited myotube formation.

Conclusions:

  • Cycloleucine effectively reduces m6A levels in poultry myoblast cells.
  • Cycloleucine treatment inhibits myoblast proliferation and myotube formation, impacting poultry muscle development.
Keywords:
N6-MethyladenosinechickencycloleucinemRNA-Seqmyogenesis

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