Application of response surface modeling optimization in UV spectrophotometric determination of 4-aminobenzoic acid...

Selective determination of 4-aminobenzoic acid (ABA) in pharmaceutical and human serum samples was performed by molecularly imprinted polypyrrole and ultraviolet (UV) spectrophotometry, based on precipitation polymerization. The molecularly imprinted polymer (MIP) was prepared using pyrrole functional monomer and ABA template molecules. The significant factors controlling the performance of the synthesized ABAMIP sorbent were screened and optimized using Plackett- Burman design (PBD) and central composite design (CCD), respectively. The model was used to obtain the optimal values of the significant response factors. The predicted MIP to NIP response ratio demonstrated an approximate deviation of 5 % from the experimental value. Under the obtained optimal conditions, the calibration curve showed a linear range of 0.05-2 mM with a correlation coefficient (r²) of 0.9920 and a limit of detection (LOD) of 0.0310 mM. The method recovery for the analyte was obtained 88.10-100.5 in the investigated real samples. The proposed ABA-MIP sorbent showed an acceptable selectivity in the presence of some pharmaceuticals.