Related Experiment Video
Updated: Aug 23, 2025
05:43
Preparation of Giant Vesicles Encapsulating Microspheres by Centrifugation of a Water-in-oil Emulsion
Published on: January 24, 2017
14.6K
Engineered multicompartment vesicosomes for selective uptake by living cells.
Vasiliy S Chernyshev1, Daniil Nozdriukhin2, Roman Chuprov-Netochin3
1Center for Photonic Science and Engineering, Skolkovo Institute of Science and Technology, 121205 Moscow, Russia; MIPT Life Sciences Center, Department of Biological and Medical Physics, Moscow Institute of Physics and Technology, 141701 Dolgoprudny, Russia.
Colloids and Surfaces. B, Biointerfaces
|October 29, 2022
Summary
Small extracellular vesicles (sEVs) enhance cell uptake of microparticles. This research reveals sEV membrane receptors
Area of Science:
- Biotechnology
- Nanomedicine
- Cell Biology
Background:
- Small extracellular vesicles (sEVs) show promise for therapy and diagnostics.
- Understanding sEV-cell interactions is crucial but underexplored.
Purpose of the Study:
- To engineer novel multicompartment particles (vesicosomes) using sEVs.
- To investigate the role of sEVs in enhancing particle uptake by cells.
Main Methods:
- Deposited sEVs from various sources onto polyelectrolyte-coated silica microparticles.
- Assessed the cellular uptake of engineered vesicosomes compared to control microparticles.
Main Results:
- sEV coating significantly enhanced the uptake of microparticles by parent cells.
- This enhancement was independent of the specific polyelectrolyte layer used.
Conclusions:
- sEV membrane receptors play a key role in mediating sEV-cell interactions.
- Vesicosomes represent a promising platform for therapeutic delivery systems.
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