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Determination of the Excitation and Coupling Rates Between Light Emitters and Surface Plasmon Polaritons
Published on: July 21, 2018
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Flexible hyperspectral surface plasmon resonance microscopy
Ziwei Liu1,2, Jingning Wu1,2, Chen Cai1,2
1State Key Laboratory of Transducer Technology, Aerospace Information Research Institute, Chinese Academy of Sciences, Beijing, 100190, China.
Nature Communications
|October 30, 2022
Summary
A new hyperspectral surface plasmon resonance microscopy (HSPRM) system offers advanced visualization and quantification of chemical and biological analytes. This breakthrough enables precise measurement of thin films, protein adsorption, and cell adhesion with high resolution.
Area of Science:
- Optics and Photonics
- Biophysics
- Materials Science
Background:
- Optical techniques are crucial for visualizing and quantifying chemical and biological analytes.
- Surface Plasmon Resonance (SPR) microscopy offers label-free detection capabilities.
- Existing SPR systems may have limitations in spectral range, resolution, or quantification accuracy.
Purpose of the Study:
- To develop and demonstrate a novel Hyperspectral Surface Plasmon Resonance Microscopy (HSPRM) system.
- To enable advanced visualization and quantification of chemical and biological samples.
- To overcome limitations of conventional SPR techniques.
Main Methods:
- Integration of a hyperspectral microscope with a prism-based spectral SPR sensor.
- Implementation of monochromatic, polychromatic SPR imaging, and single-pixel spectral SPR sensing.
- Pixel-by-pixel calibration of incident angle for SPR sensitivity correction.
Main Results:
- Demonstrated 2D quantification of thin films using resonance-wavelength images.
- Successfully quantified monolayer graphene thickness distribution.
- Showcased capabilities in analyzing inhomogeneous protein adsorption and single-cell adhesion.
Conclusions:
- The HSPRM system represents an innovative breakthrough in SPR sensor technology.
- The system offers a wide spectral range (400–1000 nm), adjustable field of view, and high lateral resolution (1.2 μm).
- HSPRM provides a powerful platform for precise chemical and biological analysis.
