Kingella pumchi sp. nov., an organism isolated from human vertebral puncture tissue

A Gram-negative, non-motile rod and strictly aerobic bacterium, designated as 18B16333^T, was isolated from vertebral puncture tissue of a patient at Peking union medical college hospital in China. Growth occurred in NaCl concentrations of 0-1% (w/v) (optimum growth at 0% NaCl), at temperatures of 25-40 °C (optimum growth at 37 °C) and at pH 6.0-9.0 (optimum growth at pH 8.0). Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine were the predominant polar lipids, and the major fatty acids were C_16:0, C_18:1 ω7c/C_18:1 ω6c and C_16:1 ω7c/C_16:1 ω6c. Phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain 18B16333^T was most closely related to Kingella potus CCUG 49773 ^T (97.3%, 16S rRNA gene sequence identity) and Neisseria bacilliformis CCUG 50858 ^T (96.8%). The ANI values between strain 18B16333^T and the type strains K. potus CCUG 49773 ^T, N. bacilliformis CCUG 50858 ^T, Kingella kingae CCUG 352 ^T and Neisseria gonorrhoeae CCUG 26876 ^T were 77.3%, 79.1%, 72.1% and 75.4%, respectively. The dDDH values between strain 18B16333^T and the four reference strains mentioned above were 24.8%, 26.9%, 24.2% and 20.7%. Further core gene analysis distinctively clustered strain 18B16333^T with four Kingella species but not with Neisseria species. Based on the phenotypic, chemotaxonomic, and phylogenetic properties, strain 18B16333^T represents a novel species of the genus Kingella, for which the name Kingella pumchi sp. nov. is proposed. The type strain is Kingella pumchi 18B16333^T (= CICC 24913 ^T = CCUG 75125 ^T).