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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

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Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
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Related Experiment Video

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Highly Multiplexed, Super-resolution Imaging of T Cells Using madSTORM
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Engineered fast-dissociating antibody fragments for multiplexed super-resolution microscopy.

Qianli Zhang1, Akitoshi Miyamoto1, Shin Watanabe1

  • 1Laboratory of Single-Molecule Cell Biology, Kyoto University Graduate School of Biostudies, Kyoto 606-8501, Japan.

Cell Reports Methods
|October 31, 2022
PubMed
Summary

Researchers developed a new method to create fluorescent probes for super-resolution microscopy. This technique simplifies the generation of fast-exchangeable probes, enabling easier multiplexed imaging of biological targets.

Failed At:

2026-06-19T13:48:56.877975+00:00

Keywords:
Fv-claspIRISantibody engineeringfast-dissociating probemultiplexed imagingmutagenesisnanobodysuper-resolution microscopysynaptic connection

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