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Updated: Aug 23, 2025
Single-Molecule Fluorescence Visualization of DNA Polymerase Dynamics at G-Quadruplexes
Published on: April 4, 2025
Exploring the fluorescence enhancement of the split G-quadruplex towards DNA-templated AgNCs and their application in
Li Yin1, Hui Zhang1, Ying Wang1
1College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, China. lulihua2012@qau.edu.cn.
Abstract:
Herein, the fluorescent enhancement of the split G-quadruplex (G4) towards DNA-templated AgNCs (DNA-AgNCs) was systematically studied. It was shown that the two G-rich probes with the ratio of the guanine (G) bases of 6 : 6 was the best split mode, and the spacer length of the zero T base at both two sides could present the optimal distance between the AgNCs and split G4. In light of these results, a split G4 coupled with DNA-AgNCs for omethoate (OME) detection was successfully fabricated. The developed fluorescent aptasensor could sense OME in the concentration range of 5-200 nM with a detection limit of 4.16 nM. Moreover, the aptasensor performed well in real-sample detection.
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