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Related Concept Videos

Caspases01:24

Caspases

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Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...
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Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Updated: Aug 23, 2025

Exploring Caspase Mutations and Post-Translational Modification by Molecular Modeling Approaches
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Exploring Caspase Mutations and Post-Translational Modification by Molecular Modeling Approaches

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Exploring Caspase Mutations and Post-Translational Modification by Molecular Modeling Approaches.

Dmitry K Nilov1, Alexey V Zamaraev2, Boris Zhivotovsky3

  • 1Belozersky Institute of Physicochemical Biology, Lomonosov Moscow State University; nilovdm@gmail.com.

Journal of Visualized Experiments : Jove
|October 31, 2022
PubMed
Summary

Molecular modeling offers a way to study caspase mutations and their effects on apoptosis without altering protein structure. This approach helps understand how post-translational modifications impact caspase function and cellular health.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Structural Biology

Background:

  • Apoptosis, or programmed cell death, is crucial for multicellular organism development and tissue homeostasis.
  • Caspases, cysteine proteases, are central to initiating and executing apoptosis.
  • Post-translational modifications dynamically regulate caspase maturation and activity.

Purpose of the Study:

  • To address limitations of traditional mutation studies in assessing post-translational modifications on caspases.
  • To present a molecular modeling protocol for evaluating amino acid substitution effects on caspase structure and function.

Main Methods:

  • Utilizing the biomolecular simulation package (Amber) for molecular modeling.
  • Employing supercomputer facilities for computational analysis.
  • Modeling both wild-type and mutant caspase forms.

Main Results:

  • The study provides a protocol to model caspase wild-type and mutant forms.
  • This method allows for the assessment of mutation impacts on protein structure and function.
  • It offers an alternative to potentially disruptive residue substitutions.

Conclusions:

  • Molecular modeling provides a viable strategy to investigate the effects of amino acid substitutions on caspases.
  • This approach avoids the conformational and functional disturbances caused by traditional mutation techniques.
  • The protocol facilitates a deeper understanding of caspase regulation and its role in apoptosis.