Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

17.3K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.3K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

142
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
142
CRISPR01:59

CRISPR

52.7K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
52.7K
DNA Bacteriophages01:26

DNA Bacteriophages

99
Bacteriophages, or phages, are viruses that specifically infect bacteria, utilizing their genetic material to hijack host cellular machinery for replication. DNA bacteriophages employ single-stranded DNA (ssDNA) or double-stranded DNA (dsDNA) genomes. These phages exhibit diverse replication strategies and host interactions, influencing their ecological roles and applications in biotechnology and medicine.ssDNA BacteriophagesssDNA phages, with their small genomes, utilize unique strategies to...
99
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

95
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
95

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Retraction: In vivo NCL targeting affects breast cancer aggressiveness through miRNA regulation.

The Journal of experimental medicine·2026
Same author

END nucleases are antiphage defence systems targeting multiple phages with modified genomes.

Nature microbiology·2026
Same author

CBASS limits bacteriophage production while maintaining cell viability in <i>Pseudomonas aeruginosa</i>.

bioRxiv : the preprint server for biology·2026
Same author

Translation-dependent degradation of cas12 mRNA triggered by an anti-CRISPR.

Nature·2026
Same author

Adaptive weight estimation and segmentation of beef carcass tissues from computed tomography images.

Meat science·2026
Same author

SwitchNet: Adaptive Distribution Switching in UNet for Brain Lesion Segmentation.

IEEE journal of biomedical and health informatics·2026

Related Experiment Video

Updated: Aug 23, 2025

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
12:21

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Published on: January 7, 2019

13.5K

Bacteriophage genome engineering with CRISPR-Cas13a.

Jingwen Guan1, Agnès Oromí-Bosch2, Senén D Mendoza1,3

  • 1Department of Microbiology & Immunology, University of California, San Francisco, CA, USA.

Nature Microbiology
|November 1, 2022
PubMed
Summary

Scientists developed new genetic engineering tools for jumbo phages using RNA-targeting CRISPR-Cas13a. This breakthrough enables easier study of these large viruses, potentially unlocking their use as antimicrobials and in basic research.

More Related Videos

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.2K
Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

22.0K

Related Experiment Videos

Last Updated: Aug 23, 2025

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
12:21

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Published on: January 7, 2019

13.5K
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

34.2K
Genome Editing in Mammalian Cell Lines using CRISPR-Cas
07:56

Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

22.0K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Jumbo phages, like Pseudomonas aeruginosa ФKZ, are promising antimicrobials and research models.
  • Genetic engineering of these phages is hindered by a protective 'phage nucleus' structure.
  • Existing DNA-targeting CRISPR-Cas systems are ineffective against this structure.

Purpose of the Study:

  • To develop novel reverse-genetics tools for DNA jumbo phages.
  • To overcome limitations posed by the phage nucleus structure.
  • To enable genetic manipulation of phages resistant to DNA-targeting systems.

Main Methods:

  • Combined homologous recombination with an RNA-targeting CRISPR-Cas13a enzyme.
  • Utilized an anti-CRISPR gene (acrVIA1) as a selectable marker.
  • Applied the system to Pseudomonas aeruginosa ФKZ and two other resistant phages.

Main Results:

  • Successfully inserted foreign genes, deleted genes, and added fluorescent tags in the ФKZ genome.
  • Demonstrated fluorescent tagging of gp93 and deletion of PhuZ, revealing insights into phage biology.
  • Achieved genome editing in two additional phages resistant to DNA-targeting CRISPR-Cas.

Conclusions:

  • RNA-targeting Cas13a provides a versatile tool for genetic engineering of intractable phages.
  • This method facilitates the study of phage genes with unknown functions.
  • Opens new avenues for utilizing jumbo phages as antimicrobials and research models.