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Multi-step Preparation Technique to Recover Multiple Metabolite Compound Classes for In-depth and Informative Metabolomic Analysis
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Comprehensive lipidomic profiling by plasma separation cards.

Lauren M Bishop1,2, Oliver Fiehn3,4

  • 1Department of Chemistry, University of California Davis, Davis, CA, USA.

Analytical and Bioanalytical Chemistry
|November 1, 2022
PubMed
Summary
This summary is machine-generated.

Plasma separation cards (PSCs) offer a promising, less-invasive method for blood lipidomic analysis. While showing strong correlation with traditional methods, lipid stability at room temperature remains a challenge for PSCs and dried blood spots.

Keywords:
LipidomicsMass spectrometryMethod validationMicrosampling

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Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Clinical Diagnostics

Background:

  • Large-scale lipidomic analyses are crucial for understanding metabolic diseases but are often hindered by the cost and invasiveness of venipuncture.
  • Microsampling techniques, such as plasma separation cards (PSCs) and dried blood spots (DBS), present a more accessible and less invasive alternative for collecting blood samples.

Purpose of the Study:

  • To evaluate the utility of plasma separation cards (PSCs) for quantitative endogenous blood lipid profiling.
  • To compare the performance of PSCs against traditional wet plasma extraction and dried blood spot (DBS) methods in terms of lipid coverage, repeatability, and stability.

Main Methods:

  • Nontargeted lipidomics was performed on samples collected via PSCs, DBS, and wet plasma extraction using liquid chromatography-high resolution tandem mass spectrometry (LC-HR-MS/MS).
  • Lipids were identified and quantified using accurate mass/retention time and MS/MS spectral library matching.
  • Inter-lot repeatability was assessed using percent relative standard deviation (%RSD), and lipid stability was evaluated at room temperature over 28 days.

Main Results:

  • A total of 498 lipid compounds across 24 subclasses were annotated.
  • Median %RSD values indicated good repeatability for PSCs (14.6%), DBS (9.3%), and wet plasma (8.6%).
  • Strong correlations in lipid peak intensities were observed between wet plasma and PSCs, whereas correlations with DBS were weaker. Lipid recovery and stability were comparable between PSC and DBS, with approximately 60% of lipids remaining stable at room temperature for 28 days.

Conclusions:

  • PSCs are a viable and improved alternative to DBS for quantitative blood lipidomic analysis due to better correlation with wet plasma.
  • Despite improvements, lipid stability at room temperature remains a significant challenge for both PSC and DBS methods outside of controlled clinical settings.
  • Data transferability and comparability to standard plasma are dependent on specific lipids and lipid classes.