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Updated: Aug 23, 2025

Author Spotlight: Advancements in iPSCs and Genetic Disease Research
Published on: October 20, 2023
A protocol to induce expandable limb-bud mesenchymal cells from human pluripotent stem cells
Tomoka Takao1, Daisuke Yamada1, Takeshi Takarada1
1Department of Regenerative Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8582, Japan.
Abstract:
Here, we present a protocol for the selective differentiation of human pluripotent stem cells mimicking human developmental processes into expandable PRRX1+ limb-bud mesenchymal (ExpLBM) cells. This approach enables expansion through serial passage while maintaining capacity for chondrogenic differentiation. For complete details on the use and execution of this protocol, please refer to Yamada et al. (2021, 2022).
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