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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

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Related Experiment Video

Updated: Aug 23, 2025

Author Spotlight: High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

Author Spotlight: High-Throughput Image-Based Quantification of Mitochondrial DNA Synthesis and Distribution

Published on: May 5, 2023

CRISPR/Cas9-based coronal nanostructures for targeted mitochondria single molecule imaging.

Xuan Zhao¹, Na Na¹, Jin Ouyang¹

¹Key Laboratory of Theoretical and Computational Photochemistry, College of Chemistry, Beijing Normal University Beijing 100875 China jinoyang@bnu.edu.cn.

Chemical Science

|November 2, 2022

Summary

This study introduces a novel DNA nanoprobe (PDNC) for precise mitochondrial RNA detection in living cells. This method enhances probe delivery and enables sensitive imaging for disease diagnosis and subcellular research.

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Area of Science:

Molecular Biology
Cell Biology
Biotechnology

Background:

Subcellular biological states, particularly mitochondrial function, are critical in disease pathogenesis.
Current nanoprobes for mitochondrial RNAs (mitomiRs) lack spatial selectivity, hindering efficient detection and signal activation.
Mitochondria play a vital role in cellular health and disease, making their monitoring essential.

Purpose of the Study:

To develop a novel DNA nanoprobe for spatially selective imaging of mitomiRs in living cells.
To improve the delivery efficiency of Cas protein for enhanced detection of mitochondrial RNAs.
To enable highly sensitive detection of low-abundance mitomiRs using a CRISPR/Cas system.

Main Methods:

Construction of a protein delivery nano-corona (PDNC) DNA nanoprobe.
Utilizing a CRISPR/Cas system for signal activation and spatially selective imaging.
Integration with a single-molecule counting method for enhanced sensitivity.

Main Results:

The PDNC nanoprobe demonstrated improved delivery efficiency of Cas protein.
Spatially selective imaging of mitomiRs in living cells was achieved.
Highly sensitive detection of low-abundance mitomiRs was enabled by the combined strategy.

Conclusions:

The developed PDNC strategy offers a novel approach for precise mitomiR detection.
This method enhances spatial selectivity and sensitivity for subcellular analysis.
The strategy holds potential for cell identification, early clinical diagnosis, and subcellular biological research.