Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

3.1K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
3.1K
Mitochondrial Membranes01:45

Mitochondrial Membranes

11.9K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
11.9K
Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

3.2K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
3.2K
Bone Remodeling01:40

Bone Remodeling

38.5K
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
38.5K
Mitochondria01:37

Mitochondria

14.2K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
14.2K
The Inner Mitochondrial Membrane01:28

The Inner Mitochondrial Membrane

3.5K
The inner mitochondrial membrane is the primary site of ATP synthesis. The inner membrane domain that forms a smooth layer adjacent to the outer membrane is called the inner boundary membrane. This domain contains membrane transporters that drive metabolites in and out of the mitochondria.  In contrast, the inner membrane network that invaginates into the matrix space is called the cristae membrane. This domain accounts for principle mitochondrial function as it accommodates the protein...
3.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

MitoSafe hypothesis: safeguarding mitochondrial morphology and innate immunity.

Trends in cell biology·2026
Same author

Genetic disruption of mitochondrial dynamics and stasis leads to liver injury and tumorigenesis.

The Journal of clinical investigation·2025
Same author

AGPAT2 acts at the crossroads of lipid biosynthesis and DRP1-mediated ER morphogenesis.

Nature communications·2025
Same author

Context-dependent inhibitory roles of RhoA in 3D invasive cell migration within the extracellular matrix.

Cell reports·2025
Same author

Targeting mitochondrial structure and dynamics for therapeutic intervention in cancer.

PLoS biology·2025
Same author

Mitochondrial fission controls astrocyte morphogenesis and organization in the cortex.

The Journal of cell biology·2025

Related Experiment Video

Updated: Aug 23, 2025

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro
07:03

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro

Published on: June 16, 2022

6.5K

Opa1-mediated mitochondrial dynamics is important for osteoclast differentiation.

Keizo Nishikawa1,2,3, Hina Takegami1, Hiromi Sesaki4

  • 1Laboratory of Cell Biology and Metabolic Biochemistry, Department of Medical Life Systems, Graduate School of Life and Medical Sciences, Doshisha University.

Micropublication Biology
|November 2, 2022
PubMed
Summary

Opa1 protein is essential for osteoclast differentiation and mitochondrial health. Opa1 deficiency impairs osteoclast formation and cristae structure, highlighting its role in mitochondrial dynamics.

More Related Videos

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes
11:52

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes

Published on: January 27, 2023

3.4K
A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation
09:37

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation

Published on: March 15, 2018

13.3K

Related Experiment Videos

Last Updated: Aug 23, 2025

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro
07:03

A Simple Pit Assay Protocol to Visualize and Quantify Osteoclastic Resorption In Vitro

Published on: June 16, 2022

6.5K
Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes
11:52

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes

Published on: January 27, 2023

3.4K
A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation
09:37

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation

Published on: March 15, 2018

13.3K

Area of Science:

  • Mitochondrial biology
  • Cell biology
  • Bone biology

Background:

  • Opatic atrophy 1 (Opa1) is a mitochondrial GTPase.
  • Opa1 regulates mitochondrial fusion and cristae architecture.
  • Osteoclasts are cells rich in mitochondria, but Opa1's role in them is unknown.

Purpose of the Study:

  • To investigate the role of Opa1 in osteoclast differentiation and function.
  • To understand how Opa1 impacts mitochondrial dynamics in osteoclasts.

Main Methods:

  • Generated Opa1-deficient osteoclast precursor cells.
  • Analyzed osteoclast differentiation efficiency.
  • Examined mitochondrial morphology, specifically cristae structure.

Main Results:

  • Opa1-deficient osteoclast precursor cells showed impaired differentiation.
  • Abnormal mitochondrial cristae morphology was observed in these cells.
  • Opa1 is crucial for maintaining mitochondrial integrity during osteoclastogenesis.

Conclusions:

  • Opa1 is a key regulator of osteoclast differentiation.
  • Opa1's function in mitochondrial dynamics is vital for osteoclast development.
  • Targeting Opa1 may offer therapeutic strategies for bone-related disorders.