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Updated: Aug 23, 2025
Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
Published on: June 8, 2020
A multicomponent reaction platform towards multimodal near-infrared BODIPY dyes for STED and fluorescence lifetime
Antonio Fernandez1,2, Nicola Kielland1,3, Ashraff Makda4
1Centre for Inflammation Research, The University of Edinburgh Edinburgh UK marc.vendrell@ed.ac.uk.
Abstract:
We report a platform combining multicomponent reaction synthesis and automated cell-based screening to develop biocompatible NIR-BODIPY fluorophores. From a library of over 60 fluorophores, we optimised compound NIRBD-62c as a multimodal probe with suitable properties for STED super-resolution and fluorescence lifetime imaging. Furthermore, we employed NIRBD-62c for imaging trafficking inside cells and to examine how pharmacological inhibitors can alter the vesicular traffic between intracellular compartments and the plasma membrane.
