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Updated: Aug 23, 2025

Murine Superficial Lymph Node Surgery

Murine Superficial Lymph Node Surgery

Published on: May 21, 2012

Sentinel node restoration by vascularized lymph node transfer in mice.

Erika G Kusajima¹, Yuhei Yamamoto¹, Kosuke Ishikawa¹

¹Department of Plastic and Reconstructive Surgery, Faculty of Medicine and Graduate School of Medicine, Hokkaido University, Sapporo, Japan.

|November 2, 2022

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Vascularized lymph node transfer (VLNT) in mice improved lymphatic function and increased dendritic cell (DC) presence, reducing cancer metastasis. This suggests VLNT enhances anti-cancer immunity by restoring lymphatic flow and DC migration.

Area of Science:

Immunology
Surgical Oncology
Regenerative Medicine

Background:

Vascularized lymph node transfer (VLNT) shows promise for impaired immunity in lymphedema.
Its impact on anti-cancer immunity and dendritic cell (DC) dynamics remains unexplored.
Early tumor immune responses involve DCs in local lymphatic recognition and antigen presentation.

Purpose of the Study:

To investigate the effect of VLNT on DC dynamics in cancer immunity.
To assess the potential of VLNT in combating cancer metastasis.
To evaluate the role of lymphatic restoration in anti-cancer immune responses.

Main Methods:

Mouse models (C57BL/6N) underwent VLNT, lymph node dissection (LND), or control procedures.
Lymphatic flow was assessed using indocyanine green lymphography.

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B16-F10 melanoma cells were implanted, and DC proportions (CD11c+) and lung metastases were quantified.

Main Results:

Lymphatic reconnection occurred in 59.2% of VLNT mice.
VLNT with lymphatic reconnection showed a significantly higher proportion of DCs (8.6%) compared to naive lymph nodes (4.3%).
The VLNT group with lymphatic reconnection exhibited significantly reduced lung metastases compared to the LND group.

Conclusions:

VLNT can decrease cancer metastasis in mice with restored lymphatic flow.
Lymphatic restoration following VLNT may enhance anti-cancer immunity by facilitating DC migration to lymph nodes.
These findings highlight VLNT as a potential therapeutic strategy for improving anti-cancer immune responses.