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Updated: Aug 23, 2025

Simple Methods for the Preparation of Non-noble Metal Bulk-electrodes for Electrocatalytic Applications

Simple Methods for the Preparation of Non-noble Metal Bulk-electrodes for Electrocatalytic Applications

Published on: June 21, 2017

A versatile artificial metalloenzyme scaffold enabling direct bioelectrocatalysis in solution.

Xiaoti Yang^1,2, Wenjie Wu^1,2, Xiling Chen³

¹Beijing National Laboratory for Molecular Science, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.

Science Advances

|November 2, 2022

Summary

Artificial metalloenzymes (ArMs) now directly catalyze electrochemical water oxidation using bacterial small laccase (SLAC) scaffolds. This breakthrough enables efficient bioelectrocatalysis for broader applications.

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Area of Science:

Bioinorganic Chemistry
Electrocatalysis
Protein Engineering

Background:

Artificial metalloenzymes (ArMs) typically use buried pockets, limiting heterogeneous catalysis.
Interfacial electron transfer in traditional ArMs is hindered by protein backbones.
A need exists for ArMs capable of direct electrochemical transformations.

Purpose of the Study:

To develop a novel protein scaffold for artificial metalloenzymes.
To enable direct electrochemical catalysis of water oxidation.
To explore the versatility of this new scaffold for bioelectrocatalysis.

Main Methods:

Utilized bacterial small laccase (SLAC) as a novel protein scaffold.
Employed molecular dynamics, X-ray crystallography, spectroscopy, and computation.
Assembled an oxo-bridged dicobalt motif on the SLAC protein surface.

Main Results:

Demonstrated direct electrochemical water oxidation catalyzed by the SLAC-based ArM in aqueous solution.
Revealed the SLAC scaffold's role in directing the four-electron water-to-oxygen transfer pathway.
Showcased successful bioelectrocatalysis of water electrolysis using various metal ions (Ni, Mn, Ru, Pd, Ir) with SLAC-ArMs.

Conclusions:

Bacterial small laccase is a viable scaffold for creating artificial metalloenzymes for direct electrocatalysis.
This approach overcomes limitations of traditional ArMs in heterogeneous transformations.
Provides a versatile platform for expanding the applications of artificial metalloenzymes in bioelectrocatalysis.