Redefining methods for augmenting lactic acid bacteria robustness and phenyllactic acid biocatalysis: Integration...

Area of Science:

• Biotechnology and Industrial Microbiology
• Metabolic Engineering
• Food Science

Background:

• Phenyllactic acid (PLA) production by lactic acid bacteria (LAB) is established, but methods for significantly increasing yields are underexplored.
• Existing research lacks a cohesive strategy for integrating simple techniques to optimize LAB metabolism for augmented PLA biosynthesis.

Purpose of the Study:

• To review and propose an orchestrated assembly of simple techniques for enhancing phenyllactic acid (PLA) production.
• To provide a preliminary guide for developing strategic experimental plans for industrial-scale PLA yields using optimized LAB metabolism.

Main Methods:

• Selection of robust, naturally isolated PLA-producing LAB strains with desirable growth and metabolic properties.
• Enhancement of LAB biotransformation potential through cryogel entrapment and/or co-cultivation strategies.
• Application of adaptive evolution to develop stress-tolerant LAB strains and optimization of fermentation parameters (temperature, pH, dissolved oxygen) and downstream processing for maximal PLA recovery.

Main Results:

• The review outlines a sequential approach to tailor LAB growth and metabolism for improved PLA catalysis.
• Integration of strain selection, novel cultivation methods, adaptive evolution, and process monitoring can significantly augment PLA production.

Conclusions:

• An orchestrated combination of simple, integrated techniques offers a resource-efficient pathway to industrial-scale phenyllactic acid production.
• This review provides a strategic framework for optimizing LAB metabolism to achieve substantial PLA yields, conserving time, effort, and resources.