SERS of cylindrospermopsin cyanotoxin: Prospects for quantitative analysis in solution and in fish tissue

Cylindrospermopsin (CYN), a cyanotoxin occurring in environmental waters as a cyanobacteria metabolite, has recently raised increased interest both in the scientific community and the environmental, food control and health care bodies due to the incidence of poisoning reports and the lack of prompt, effective detection and monitoring techniques. Here we report comprehensive Raman and SERS spectroscopy data on CYN cyanotoxin and provide a detailed characterization of the vibrational Raman signal based on DFT calculation as well as the adsorption properties with respect to the silver nanoparticles surface. Quantitative SERS analysis was achieved for concentrations range from 0.218 nM to 2.18 µM in aqueous solution. We further investigated the SERS discrimination of artificially intoxicated fish tissue from normal one, using linear discriminant analysis. Significant changes in SERS signal of toxic tissue compared to normal one allowed clear and fast differentiation of toxic tissue with 100% specificity/sensitivity. The cross-validation procedure provided 100% clear separation based on the SERS data. The results open reliable perspectives for SERS monitoring the environmental water bodies.